Difference between revisions of "Part:BBa M36143:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Precursor (Pro EP-B2) and mature EBp-B2 in order to test if inhibitor is necessary for survival of cells. | |
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===Source=== | ===Source=== | ||
Latest revision as of 22:57, 22 October 2015
Inhibitor I29 Attached to Cysteine Proteinase EP-B2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 33
Illegal BglII site found at 702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 370
Illegal AgeI site found at 454
Illegal AgeI site found at 793 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Precursor (Pro EP-B2) and mature EBp-B2 in order to test if inhibitor is necessary for survival of cells.
Source
DNA sequence for precursor and mature EP-B2 generated from amino acid sequence in "Computational Design of an α-Gliadin Peptidase".