Difference between revisions of "Part:BBa K1741013"
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https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg | https://static.igem.org/mediawiki/parts/b/ba/UAMpoznanproChairpin.jpg | ||
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+ | This is the potential hairpin sequence. | ||
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+ | ===Results=== | ||
+ | |||
+ | Legend: | ||
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+ | proC [[https://parts.igem.org/Part:BBa_K1741012 BBa_K1741012]] | ||
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+ | proC with a hairpin in 5'UTR [[https://parts.igem.org/Part:BBa_K1741013 BBa_K1741013]] | ||
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+ | proD [[https://parts.igem.org/Part:BBa_K1741014 BBa_K1741014]] | ||
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+ | https://static.igem.org/mediawiki/parts/3/35/Teamuamprocdiffsugarsgraph.png | ||
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+ | https://static.igem.org/mediawiki/parts/8/86/Teamuampoznanprochairpindiffsuagrsgraph.png | ||
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+ | https://static.igem.org/mediawiki/parts/d/df/Teamuampoznanproddiffsugarsgraph.png | ||
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+ | The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. | ||
+ | |||
+ | We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C. | ||
+ | |||
Revision as of 07:33, 27 September 2015
sfGFP under proC promoter with a hairpin in 5'UTR
A weak hairpin introduced to 5’UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.
This is the potential hairpin sequence.
Results
Legend:
proC [BBa_K1741012]
proC with a hairpin in 5'UTR [BBa_K1741013]
proD [BBa_K1741014]
The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairspin to stop the translation in 23° C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI.rc site found at 232