Difference between revisions of "Part:BBa K1741003"

(→‎Characteristics and Results)
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<partinfo>BBa_K1741003 short</partinfo>
 
<partinfo>BBa_K1741003 short</partinfo>
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==Design==
  
 
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.  
 
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.  
  
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===Results===
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Legend:
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MelS [[https://parts.igem.org/Part:BBa_K1741004 BBa_K1741004]]
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https://static.igem.org/mediawiki/parts/f/f1/UAMpoznanmelS.jpg
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MelWT [[https://parts.igem.org/Part:BBa_K1741003 BBa_K1741003]]
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https://static.igem.org/mediawiki/parts/0/02/UAMpoznanmelWT.jpg
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===Characteristic and Results===
  
  
 
Legend:
 
Legend:
  
−
MelS [[https://parts.igem.org/Part:BBa_K1741004 Ba_K1741004]]
 
  
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MelWT [[https://parts.igem.org/Part:BBa_K1741003 Ba_K1741003]]
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MelS [[https://parts.igem.org/Part:BBa_K1741004 BBa_K1741004]]
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https://static.igem.org/mediawiki/parts/f/f1/UAMpoznanmelS.jpg
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MelWT [[https://parts.igem.org/Part:BBa_K1741003 BBa_K1741003]]
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https://static.igem.org/mediawiki/parts/0/02/UAMpoznanmelWT.jpg
  
 
https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
 
https://static.igem.org/mediawiki/parts/9/97/Teamuampoznanmelibiosem9graph.png
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https://static.igem.org/mediawiki/parts/2/2c/Teamuampoznanmelibioselbgraph.png
 
https://static.igem.org/mediawiki/parts/2/2c/Teamuampoznanmelibioselbgraph.png
  

Revision as of 06:57, 27 September 2015

sfGFP under melibiose promoter

Design

MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.

Legend:

MelS [BBa_K1741004]

UAMpoznanmelS.jpg

MelWT [BBa_K1741003]

UAMpoznanmelWT.jpg

Characteristic and Results

Legend:


MelS [BBa_K1741004]

UAMpoznanmelS.jpg

MelWT [BBa_K1741003]

UAMpoznanmelWT.jpg

Teamuampoznanmelibiosem9graph.png

Teamuampoznanmelibioselbgraph.png


The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We also checked the tightness of our promoters.

All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose. Teamuampoznanpromoterscomparisongrpah.png




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 206