Difference between revisions of "Part:BBa K1741003"
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The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. | The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter. | ||
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| + | We also checked the tightness of our promoters. | ||
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| + | All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose. | ||
| + | https://static.igem.org/mediawiki/parts/4/43/Teamuampoznanpromoterscomparisongrpah.png | ||
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Revision as of 06:41, 27 September 2015
sfGFP under melibiose promoter
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.
Characteristics and Results
Legend:
MelS [Ba_K1741004]
MelWT [Ba_K1741003]
The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
We also checked the tightness of our promoters.
All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 154
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 206