Difference between revisions of "Part:BBa K1033250:Experience"
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===Applications of BBa_K1033250=== | ===Applications of BBa_K1033250=== | ||
| − | Utah State used this fluorescent protein in their 2015 for a project engineering ''Lactococcus lactis'' to detect and fight against phi31 phage infection. It was not tested in ''L. lactis'' but did display function in ''E. coli'' when paired with three ''L. lactis'' promoters. (see | + | Utah State used this fluorescent protein in their 2015 for a project engineering ''Lactococcus lactis'' to detect and fight against phi31 phage infection. It was not tested in ''L. lactis'' but did display function in ''E. coli'' when paired with three ''L. lactis'' promoters. (see Figures 1 and 2). |
https://static.igem.org/mediawiki/2015/0/0b/Utah_State_2015_MCherry_Fluorescence_Chart_Version_2.jpg | https://static.igem.org/mediawiki/2015/0/0b/Utah_State_2015_MCherry_Fluorescence_Chart_Version_2.jpg | ||
<p>Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 530/25 with emissions read at 590/35. cp8 from part BBa_K1820017, cp11 from part BBa_K1820018, and cp44 from part BBa_K1820019</p> | <p>Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 530/25 with emissions read at 590/35. cp8 from part BBa_K1820017, cp11 from part BBa_K1820018, and cp44 from part BBa_K1820019</p> | ||
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| + | https://static.igem.org/mediawiki/parts/d/de/Utah_State_2015_Fluorescence_small.jpg | ||
| + | <p>Figure 2. Fluorescence ''E.coli'' bacteria with ''Lactococcus lactis'' promoters and sfGFP(Bs) (left) and mCherry(Lr) (right) | ||
<p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/ </p> | <p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/ </p> | ||
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Applications of BBa_K1033250
Utah State used this fluorescent protein in their 2015 for a project engineering Lactococcus lactis to detect and fight against phi31 phage infection. It was not tested in L. lactis but did display function in E. coli when paired with three L. lactis promoters. (see Figures 1 and 2).
Figure 1. Fluorescence levels from three constructs using synthetic promoters from Jensen and Hammer (1998) in E. coli excited at 530/25 with emissions read at 590/35. cp8 from part BBa_K1820017, cp11 from part BBa_K1820018, and cp44 from part BBa_K1820019
Figure 2. Fluorescence E.coli bacteria with Lactococcus lactis promoters and sfGFP(Bs) (left) and mCherry(Lr) (right) <p> Referrence: Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/
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