Difference between revisions of "Part:BBa K1783003:Experience"
(→‎Applications of BBa_K1783003)
Line 5: Line 5:
 
===Applications of BBa_K1783003===
 
===Applications of BBa_K1783003===
  
−
Uncomplicated transformation and cloning.  
+
Uncomplicated transformation.  
−
Expression of RFP may differ based on strain of <i>E. Coli</i>. According to experimentation, DH5α strain may more optimally express the protein of interest than BL21.  
+
Expression of RFP may differ based on strain of <i>E.coli</i>. According to experimentation, DH5α strain may more optimally express the protein of interest than BL21 strain.
 +
 
 +
The below graph is testing the plating of this construct in DH5α:
 +
 
 +
[[https://static.igem.org/mediawiki/2015/d/d0/UMDHSplate2.png]]
 +
 
 +
The below graph is testing the fluorescence of this construct in DH5α:
 +
 
 +
[[https://static.igem.org/mediawiki/2015/d/d5/FtestHSUMD.png]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 04:24, 26 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1783003

Uncomplicated transformation. Expression of RFP may differ based on strain of E.coli. According to experimentation, DH5α strain may more optimally express the protein of interest than BL21 strain.

The below graph is testing the plating of this construct in DH5α:

[[1]]

The below graph is testing the fluorescence of this construct in DH5α:

[[2]]

User Reviews

UNIQ7ba2e41a82c4c987-partinfo-00000000-QINU UNIQ7ba2e41a82c4c987-partinfo-00000001-QINU