Difference between revisions of "Part:BBa K1783003:Design"
(→‎Design Notes)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Construct was assembled using Gibson assembly of a G-block of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a PSB1C3 backbone.  
+
Construct was assembled using Gibson assembly of a g-block of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a pSB1C3 backbone.  
−
PCR for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.  
+
PCR amplification for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.
  
 
===Source===
 
===Source===

Revision as of 04:05, 26 September 2015

Unstable RFP under Hok/Sok Regulation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 587
    Illegal NheI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct was assembled using Gibson assembly of a g-block of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a pSB1C3 backbone. PCR amplification for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.

Source

See construction parts.

References