Difference between revisions of "Part:BBa K1602044"
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Composite part consisting of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), Lock3 (<html><a href="/Part:BBa_J01080">BBa_J01080</a></html>) and hokD (<html><a href="/Part:BBa_K1497008">BBa_K1497008</a></html>). It belongs to a two-part killswitch-system for <i>E.coli</i> utillizing a riboregulator for posttransciptional regulation of hokD. Upon transcription the sequence of <html><a href="/Part:BBa_J01080">Lock3</a></html> forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence.
 
Composite part consisting of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), Lock3 (<html><a href="/Part:BBa_J01080">BBa_J01080</a></html>) and hokD (<html><a href="/Part:BBa_K1497008">BBa_K1497008</a></html>). It belongs to a two-part killswitch-system for <i>E.coli</i> utillizing a riboregulator for posttransciptional regulation of hokD. Upon transcription the sequence of <html><a href="/Part:BBa_J01080">Lock3</a></html> forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence.
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If the corresponding trans-activating RNA-sequence (taRNA) (<html><a href="/Part:BBa_J01086">Key3</a></html>) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of hokD resulting in cell death.
+
If the corresponding trans-activating RNA-sequence (taRNA) (<html><a href="/Part:BBa_J01086">Key3 BBa_J01086</a></html>) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of hokD resulting in cell death.
  
 
<html>
 
<html>
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<figure >
 
<figure >
 
<img width=30%; src="https://static.igem.org/mediawiki/parts/2/22/Schema_killswitch.png">
 
<img width=30%; src="https://static.igem.org/mediawiki/parts/2/22/Schema_killswitch.png">
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<figcaption><b>Figure 2:</b> Interaction of taRNA and crRNA leads to expression of hokD.</figcaption>
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<figcaption><b>Figure 1:</b> Interaction of taRNA and crRNA leads to expression of hokD.</figcaption>
 
</figure>
 
</figure>
 
</center>
 
</center>

Revision as of 07:05, 25 September 2015

RRL3H

Composite part consisting of a constitutive promoter (BBa_J23100), Lock3 (BBa_J01080) and hokD (BBa_K1497008). It belongs to a two-part killswitch-system for E.coli utillizing a riboregulator for posttransciptional regulation of hokD. Upon transcription the sequence of Lock3 forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following hokD-sequence. If the corresponding trans-activating RNA-sequence (taRNA) (Key3 BBa_J01086) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of hokD resulting in cell death.

Figure 1: Interaction of taRNA and crRNA leads to expression of hokD.
.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]