Difference between revisions of "Part:BBa K1602054"
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This part is half of a two-part riboregulator-system for posttransciptional regulation of GFP-expression. The part was created by inserting a GFP-gene flanked by the restriction sites BamHI and HindIII into <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html> via restriction cloning. It consists of the fused sequences of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), a cis-repressing sequence (cr), GFP (<html><a href="/Part:BBa_E0040">BBa_E0040</a></html>) and a terminator (<html><a href="/Part:BBa_B0015">BBa_B0015</a></html>). The restriction sites upstream and downstream of the GFP sequence allow for an easy exchange of the GFP gene with another gene of interest. | This part is half of a two-part riboregulator-system for posttransciptional regulation of GFP-expression. The part was created by inserting a GFP-gene flanked by the restriction sites BamHI and HindIII into <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html> via restriction cloning. It consists of the fused sequences of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), a cis-repressing sequence (cr), GFP (<html><a href="/Part:BBa_E0040">BBa_E0040</a></html>) and a terminator (<html><a href="/Part:BBa_B0015">BBa_B0015</a></html>). The restriction sites upstream and downstream of the GFP sequence allow for an easy exchange of the GFP gene with another gene of interest. | ||
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| + | Upon transcription the cis-repressing sequence forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following GFP-sequence. | ||
If the corresponding trans-activating RNA-sequence (taRNA) (<html><a href="/Part:BBa_K1602049">RRKey-BBa_K1602049</a></html>) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of GFP as the gene of interest (GOI). | If the corresponding trans-activating RNA-sequence (taRNA) (<html><a href="/Part:BBa_K1602049">RRKey-BBa_K1602049</a></html>) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of GFP as the gene of interest (GOI). | ||
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Revision as of 06:47, 25 September 2015
RRGFP
This part is half of a two-part riboregulator-system for posttransciptional regulation of GFP-expression. The part was created by inserting a GFP-gene flanked by the restriction sites BamHI and HindIII into RRlocked_site via restriction cloning. It consists of the fused sequences of a constitutive promoter (BBa_J23100), a cis-repressing sequence (cr), GFP (BBa_E0040) and a terminator (BBa_B0015). The restriction sites upstream and downstream of the GFP sequence allow for an easy exchange of the GFP gene with another gene of interest.
Upon transcription the cis-repressing sequence forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following GFP-sequence. If the corresponding trans-activating RNA-sequence (taRNA) (RRKey-BBa_K1602049) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of GFP as the gene of interest (GOI).
Functional Parameters
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 91
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 737