Difference between revisions of "Part:BBa K1602045"
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<partinfo>BBa_K1602045 short</partinfo>
 
<partinfo>BBa_K1602045 short</partinfo>
  
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Composite part consisting of a constitutive promoter (<html><a href="/Part:BBa_J23100">BBa_J23100</a></html>), Lock3 (<html><a href="/Part:BBa_J01080">BBa_J01080</a></html>) and GFP (<html><a href="/Part:BBa_E0040">BBa_E0040</a></html>). It belongs to a two-part riboregulator-system for posttransciptional regulation of GFP-expression. Upon transcription the sequence of <html><a href="/Part:BBa_J01080">Lock3</a></html> forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following GFP-sequence.
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If the corresponding trans-activating RNA-sequence (taRNA) (<html><a href="/Part:BBa_J01086">Key3-BBa_J01086</a></html>) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of GFP as the gene of interest (GOI).
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<html>
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<center>
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<figure >
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<img width=30%; src="https://static.igem.org/mediawiki/parts/3/3d/Schema_GOI.png">
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<figcaption><b>Figure 1:</b> Interaction of taRNA and crRNA leads to expression of GFP.</figcaption>
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</figure>
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</center>
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</html>
  
 
===Functional Parameters===
 
===Functional Parameters===

Revision as of 07:14, 25 September 2015

RRL3G

Composite part consisting of a constitutive promoter (BBa_J23100), Lock3 (BBa_J01080) and GFP (BBa_E0040). It belongs to a two-part riboregulator-system for posttransciptional regulation of GFP-expression. Upon transcription the sequence of Lock3 forms a hairpin-secondary-structure masking the ribosome binding site (RBS) and therefore prevents translation of the following GFP-sequence. If the corresponding trans-activating RNA-sequence (taRNA) (Key3-BBa_J01086) is present the two sequences form a RNA-RNA-complex. This leads to a helix shift and the release of the RBS enabling the expression of GFP as the gene of interest (GOI).

Figure 1: Interaction of taRNA and crRNA leads to expression of GFP.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733