Difference between revisions of "Part:BBa K1602049"

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<partinfo>BBa_K1602049 short</partinfo>
 
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This part is half of a two-part riboregulator-system for <i>E.coli</i> for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the RBS enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are <html><a href="/Part:BBa_K1602050">RRlocked</a></html> and <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html>.  
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This part is half of a two-part riboregulator-system for <i>E.coli</i> for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are <html><a href="/Part:BBa_K1602050">RRlocked</a></html> and <html><a href="/Part:BBa_K1602053">RRlocked_site</a></html>.  
  
 
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Revision as of 03:20, 25 September 2015

RRkey

This part is half of a two-part riboregulator-system for E.coli for posttransciptional regulation of gene expression. Upon transcription the produced trans-activating RNA-sequence (taRNA) forms a RNA-RNA-complex with corresponding cis-repressing sequences (crRNA). This leads to a helix shift and the release of the formerly masked ribosome binding site (RBS) enabling the expression of the regulated gene of interest (GOI). The corresponding crRNAs are RRlocked and RRlocked_site.

Figure 1: Interaction of taRNA and crRNA leads to gene expression.

The sequence of the taRNA is based on a existing riboregulator sequence pair published by Isaacs et al[1]. The original sequence contained an EcoRI restriction site which was removed by basepair-exchange. We used the Riboswitch Designer to find out which basepair-exchange is best suited to remove the unwanted EcoRI restriction site while not affecting the folding- and interaction-capabilities of the sequence.

Functional Parameters

References

1. Isaacs, F.J., et al., Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol, 2004. 22(7): p. 841-7.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]