Difference between revisions of "Part:BBa K1616005:Experience"
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<h3><font style="color:#b22222">Ligation of Gblocks Holin-Endolysin into pDawn </font></h3> | <h3><font style="color:#b22222">Ligation of Gblocks Holin-Endolysin into pDawn </font></h3> | ||
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Tube | Tube | ||
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| − | T4 ligase | + | <b>T4 ligase</b> |
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<h2>Results </h2> | <h2>Results </h2> | ||
Revision as of 23:57, 22 September 2015
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Applications of BBa_K1616005
Notebook
Ligation of Gblocks Holin-Endolysin into pDawn
|
Tube |
H/E |
|---|---|
|
Water |
4,6 µL |
|
T4 ligase Buffer |
1 µL |
|
pDawn |
1,4 µL |
|
Plasmid |
2,5 µL |
|
T4 ligase |
0,5 µL |
Results
Bacteria expressing pDawn-HE were prepared in a pre-culture until DO600 reached a value between 0,6 and 0,8. Then, one condition was illuminated with white-light and incubated at 37°C, another one was kept in the dark and incubated at 37°C and the last condition was illuminated with white-light and incubated at room temperature. The value of DO was taken regularly to observe the kinetic of growth under each condition.
In absence of light, and at 37°C, bacteria growth was normal but light illumination was enough to slow-down the growth of bacteria. Incubation at room temperature with white-light illumination allowed better folding of HE and had a stronger effect on the growth of bacteria.
User Reviews
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