Difference between revisions of "Part:BBa K1618021"

Revision as of 02:30, 26 September 2015

Plac-THL-CRO-BHBD-BCD-EFTb-EFTa

K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA ¹. At the last moment we realised that there was an EcoRI site on the gene sequence so we're working to fix this as soon as possible.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14..

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal NheI site found at 1167
Illegal NotI site found at 235
Illegal NotI site found at 3841
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal BglII site found at 1415
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
Illegal XbaI site found at 244
Illegal AgeI site found at 1338
Illegal AgeI site found at 3354
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1618021 short</partinfo>
-K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA.
+K1618021 contains the coding sequences for the Thiolase,Crotonase, β-hydroxybutyryl-CoA Dehydrogenase,Butyryl-CoA Dehydrogenase,Electron Transfer Protein β and Electron Transfer Protein α from Coprococcus sp. (L2-50 DSM),under control of the LacI promoter. This construct builds the butyrate pathway (Figure 1) that converts acetyl-CoA into Butyry-CoA <sup>1</sup>. At the last moment we realised that there was an EcoRI site on the gene sequence so we're working to fix this as soon as possible.
+. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, <i>Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene</i> , Environmental Microbiology, 12(2) p. 304–14..
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