Difference between revisions of "Part:BBa K1840008:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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== '''1. Fluorescence after incubation in M9 minimal media and 5%, 10% or 20% glucose''' == | == '''1. Fluorescence after incubation in M9 minimal media and 5%, 10% or 20% glucose''' == | ||
Three biological replicates of gad transformed cells have been inoculated in M9 minimal media with 5%, 10% or 20% of glucose. After 16 hours incubation, 100ul of each sample was transferred to a black 96 well plate with transparent bottom. The plate reader measured the fluorescence (excitation:584nm, emission:620nm) and the OD (600nm). Three technical replicates were measured. Following figure shows the average of all technical and biological replicates with standard deviation as error bar. | Three biological replicates of gad transformed cells have been inoculated in M9 minimal media with 5%, 10% or 20% of glucose. After 16 hours incubation, 100ul of each sample was transferred to a black 96 well plate with transparent bottom. The plate reader measured the fluorescence (excitation:584nm, emission:620nm) and the OD (600nm). Three technical replicates were measured. Following figure shows the average of all technical and biological replicates with standard deviation as error bar. | ||
| − | [[File:gad-m9.png| | + | [[File:gad-m9.png|600px]] |
Cells containing the gad-device displayed and increasing expression of mCherry with increasing glucose concentration in the environment. Even though the tendency line was calculated for a linear correlation, it might be possible that the correlation between glucose concentration and mCherry is different, for example exponentially. Further measurement with concentrations in between have to be done to confirm that theory. | Cells containing the gad-device displayed and increasing expression of mCherry with increasing glucose concentration in the environment. Even though the tendency line was calculated for a linear correlation, it might be possible that the correlation between glucose concentration and mCherry is different, for example exponentially. Further measurement with concentrations in between have to be done to confirm that theory. | ||
| − | [[File:plate-m9.png| | + | [[File:plate-m9.png|600px]] |
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:13, 25 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1840008
This part can be used as a glucose sensor. It expresses mCherry according to the level of glucose in the environment. Please see the following experiments and their results for characterization:
1. Fluorescence after incubation in M9 minimal media and 5%, 10% or 20% glucose
Three biological replicates of gad transformed cells have been inoculated in M9 minimal media with 5%, 10% or 20% of glucose. After 16 hours incubation, 100ul of each sample was transferred to a black 96 well plate with transparent bottom. The plate reader measured the fluorescence (excitation:584nm, emission:620nm) and the OD (600nm). Three technical replicates were measured. Following figure shows the average of all technical and biological replicates with standard deviation as error bar.
Cells containing the gad-device displayed and increasing expression of mCherry with increasing glucose concentration in the environment. Even though the tendency line was calculated for a linear correlation, it might be possible that the correlation between glucose concentration and mCherry is different, for example exponentially. Further measurement with concentrations in between have to be done to confirm that theory.
User Reviews
UNIQbea681c899d85b2b-partinfo-00000000-QINU UNIQbea681c899d85b2b-partinfo-00000001-QINU