Difference between revisions of "Part:BBa K1859021:Experience"

Line 18: Line 18:
 
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.<br>
 
There was no fluorescence. We found that this generator doesn’t make functional long chain protein.<br>
  
 +
</html>
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K1859021 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1859021 StartReviews</partinfo>

Revision as of 09:21, 22 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859021===

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane F shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.

User Reviews

UNIQ50175d1aa3c97b05-partinfo-00000001-QINU UNIQ50175d1aa3c97b05-partinfo-00000002-QINU