Difference between revisions of "Part:BBa K1859026"
| Line 30: | Line 30: | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > [BBa_K1859016] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859016" > [BBa_K1859016] </a> | ||
), His-RFP without stop codon | ), His-RFP without stop codon | ||
| − | <a href= "https://parts.igem.org/wiki/index.php?title=Part: | + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" >[BBa_K1332002] </a> |
, Lacl | , Lacl | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
Revision as of 08:29, 22 September 2015
mRNA circularize efficiently generator (outside complementarity)
We designed this generator [K1859026] to improve circular efficiency of mRNA including His-RFP sequence.
First, we improved Circular Parts of iGEM2014,the 5´side of the intron [BBa_K1332003] and the 3´side of the intron [BBa_K1332005] , and made [BBa_K1859015] and [BBa_K1859016] . Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. However, [BBa_K1859015] and [BBa_K1859016] were cloned the sequence extra in addition to the ribozyme .
The generator is comprised of Circular parts improved to become complementary ( [BBa_K1859015] and [BBa_K1859016] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .
Tne RNA made by this generator [BBa_1859026] was designed to bind outside of circular mRNA complementary.
We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_K1859024] , [BBa_K1859025] ,[BBa_K1859026].