Difference between revisions of "Part:BBa K1615092"
LiusaidhOwen (Talk | contribs) |
LiusaidhOwen (Talk | contribs) |
||
| Line 6: | Line 6: | ||
<br> | <br> | ||
[[File:CBD RFP folding.jpg]] | [[File:CBD RFP folding.jpg]] | ||
| + | <br> | ||
| + | <br> | ||
| + | We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD. | ||
| + | <br> | ||
| + | <br> | ||
| + | [[File:BBa_K1615092.jpg]] | ||
| + | <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:23, 22 September 2015
mRFP fused to CBDcenA+Linker in RFC25
mRFP (BBa_K1615089) fused to the N terminal of CBDcenA + C terminal linker (BBa_K1321339) in RFC25. If folded properly, this allows mRFP to fluoresce while immobilised on cellulose.
We tested the dissociation of this part on Whatman 54 using isostandard chads. This involved incubating the chads in the crude cell lysate for 10 minutes and then washing them with PBS for various periods time to test the binding affinity of this CBD.
File:BBa K1615092.jpg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]