Difference between revisions of "Part:BBa K1615111"
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Cellulose binding domains (CBDs) mediate the binding of enzymes to cellulose<sup>1</sup>. CBDs are divided into over a dozen families based on their sequence homology <sup>2</sup>. Family III of CBDs is divided into a, b and c with CBDCipA belonging to family III a; the clostridial scaffoldin CBDs<sup>3</sup>. CBDCipA was identified in <i>Clostridium thermocellum</i> and is capable of binding to crystalline cellulose in a reversible manner<sup>4</sup>. CBDCipA includes endogenous linker sequences at both the N and C-terminals which help to prevent the CBD from interfering with the folding of any other protein it may be fused to. | Cellulose binding domains (CBDs) mediate the binding of enzymes to cellulose<sup>1</sup>. CBDs are divided into over a dozen families based on their sequence homology <sup>2</sup>. Family III of CBDs is divided into a, b and c with CBDCipA belonging to family III a; the clostridial scaffoldin CBDs<sup>3</sup>. CBDCipA was identified in <i>Clostridium thermocellum</i> and is capable of binding to crystalline cellulose in a reversible manner<sup>4</sup>. CBDCipA includes endogenous linker sequences at both the N and C-terminals which help to prevent the CBD from interfering with the folding of any other protein it may be fused to. | ||
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Revision as of 23:29, 21 September 2015
CBDcipA with N and C-terminal linker in RFC25
Cellulose binding domains (CBDs) mediate the binding of enzymes to cellulose1. CBDs are divided into over a dozen families based on their sequence homology 2. Family III of CBDs is divided into a, b and c with CBDCipA belonging to family III a; the clostridial scaffoldin CBDs3. CBDCipA was identified in Clostridium thermocellum and is capable of binding to crystalline cellulose in a reversible manner4. CBDCipA includes endogenous linker sequences at both the N and C-terminals which help to prevent the CBD from interfering with the folding of any other protein it may be fused to.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]