Difference between revisions of "Part:BBa K1846001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by | + | This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part [https://parts.igem.org/Part:BBa_K1846001 BBa_K1846001]. |
[[File:BBKiGEM-tfa-circuit.jpg|400px|thumb|none|]] | [[File:BBKiGEM-tfa-circuit.jpg|400px|thumb|none|]] | ||
Revision as of 00:15, 21 September 2015
Bacteriophage lambda tail fibre assembly protein circuit
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This tfa circuit was synthesised removing the illegal restriction sites to make the sequence BioBrick-compatible. The sequence was cloned into a pSB1C3 and the success of the cloning procedure was confirmed by restriction with EcoRI and PstI restriction enzymes and followed by agarose gel electrophoresis (Figure 1). The construct was also confirmed by sequencing. This BioBrick was registered as part BBa_K1846001.
Source
DNA synthesis