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Revision as of 18:58, 20 September 2015

RFC 110 forward linearization primer

Primer for the linearization of the RFC 110 standard assembly vector.

Usage and Biology

This sequence and BBa_K1614004 are the primers that you need to linearize BBa_K1614002 in order to insert the DNA template for your RNA of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters