Difference between revisions of "Part:BBa K1598005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part consists of the pYeaR promoter: | + | This part consists of the pYeaR promoter: [[BBa_K216005]], an RBS, the TPH1 expression gene: [[BBa_K1598001]] and a terminator. |
- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag | - human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag | ||
Revision as of 17:05, 20 September 2015
pYear-RBS-TPH1-6xHis-Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part consists of the pYeaR promoter: BBa_K216005, an RBS, the TPH1 expression gene: BBa_K1598001 and a terminator.
- human TPH1 gene was codon-optimized for E.coli and provided with C-terminal 6xHis tag - sequence was synthesized in the form of gBlock and cloned into pSB1C3
Source
DNA synthesis