Difference between revisions of "Part:BBa K1806004:Experience"
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Abdulkadir (Talk | contribs) (→Cloning) |
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The composite system was ligated to the cloning vector psB1C3, to be transformated and then verified with Colony PCR. The verified ligation was then moved on to the digestion step to be cut with the EcoR1 and Pst1 restriction enzymes. The gel run of the cut parts were portrayed down below. The part was tagged as G-Block 6 and sample 6-2 yielded accurate results. | The composite system was ligated to the cloning vector psB1C3, to be transformated and then verified with Colony PCR. The verified ligation was then moved on to the digestion step to be cut with the EcoR1 and Pst1 restriction enzymes. The gel run of the cut parts were portrayed down below. The part was tagged as G-Block 6 and sample 6-2 yielded accurate results. | ||
| − | [[File: | + | [[File:AUC TURKEY 6 colony.png]] [[File:6004-2.png]] |
[[File:6004-3.png]] [[File:6004-4.png]] | [[File:6004-3.png]] [[File:6004-4.png]] | ||
Revision as of 21:28, 20 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1806004
Cloning
The composite system was ligated to the cloning vector psB1C3, to be transformated and then verified with Colony PCR. The verified ligation was then moved on to the digestion step to be cut with the EcoR1 and Pst1 restriction enzymes. The gel run of the cut parts were portrayed down below. The part was tagged as G-Block 6 and sample 6-2 yielded accurate results.
User Reviews
UNIQfc431d3fcc16f295-partinfo-00000000-QINU UNIQfc431d3fcc16f295-partinfo-00000001-QINU