Difference between revisions of "Part:BBa K1614002:Design"
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===Design Notes=== | ===Design Notes=== | ||
The cassette contains a T7 promoter which enables the transcription of the RNA of Interest using the T7 RNA Polymerase. Cleavage of the RNA of Interest is achieved by adding an HDV behind the RNA of Interest. | The cassette contains a T7 promoter which enables the transcription of the RNA of Interest using the T7 RNA Polymerase. Cleavage of the RNA of Interest is achieved by adding an HDV behind the RNA of Interest. | ||
| − | [[File:HeidelbergPWiGEM2015_RFC.png|thumb|General mechanism of the RFC which is based on BioBrick BBa_K1614002]] | + | [[File:HeidelbergPWiGEM2015_RFC.png|600 px|thumb|General mechanism of the RFC which is based on BioBrick BBa_K1614002]] |
===Source=== | ===Source=== | ||
Revision as of 15:24, 20 September 2015
transcription cassette of BBF RFC 110
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 32
Illegal NgoMIV site found at 61 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cassette contains a T7 promoter which enables the transcription of the RNA of Interest using the T7 RNA Polymerase. Cleavage of the RNA of Interest is achieved by adding an HDV behind the RNA of Interest.
Source
Standard assembly construct for RFC 110 assembly