Difference between revisions of "Part:BBa K773002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part was improved by Silvia Galvan of the iGEM UniTN Trento 2015 team. Check the experience page of this part for more information. | This part was improved by Silvia Galvan of the iGEM UniTN Trento 2015 team. Check the experience page of this part for more information. | ||
| + | This part was previously found by other users to not express protein nor produce ATP. We improved this part by adding a strong RBS and using a different inducible promoter (arabinose, araC-pBAD). We were able to express the protein and functionally characterize it. The part now works! | ||
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Revision as of 15:24, 20 September 2015
Proteorhodopsin
This part carries the gene for a proteorhodopsin pump, which can generate a proton motive force for making ATP.
Usage and Biology
This part was improved by Silvia Galvan of the iGEM UniTN Trento 2015 team. Check the experience page of this part for more information. This part was previously found by other users to not express protein nor produce ATP. We improved this part by adding a strong RBS and using a different inducible promoter (arabinose, araC-pBAD). We were able to express the protein and functionally characterize it. The part now works!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 515
Illegal XhoI site found at 88 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 277
Illegal AgeI site found at 652 - 1000COMPATIBLE WITH RFC[1000]