Difference between revisions of "Part:BBa K1777008"
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We design this RNA to promote the cyclization of hsACTB in HEK293T cells.<br> | We design this RNA to promote the cyclization of hsACTB in HEK293T cells.<br> | ||
| − | [[File:hsACTB.JPG| | + | [[File:hsACTB.JPG| 900 px]] |
===Results=== | ===Results=== | ||
Revision as of 12:55, 20 September 2015
RNA accelerating cyclization
This RNA can bind to the flanking region of exon4 of hsACTB in HEK293T cells and facilitate the back-splicing mechanism. This part can be used to cyclize certain RNA exon.
Fuction
We design this RNA to promote the cyclization of hsACTB in HEK293T cells.
Results
Forty-eight hours after transfection with pZW1-sno-ACTB into HEK 293T cells, we use the specific circular exon-4 primers to test the circular RNA.
Unexpectedly, we amplify other circular RNAs using specific primers.(BLAST on UCSC-In Silico, proved to be specific）
Both vector without insert pZW1-sno and plasmid with acRNA pZW1-sno-ACTB appear non-specific electrophoretic band. After sequencing, we found 200bp: circular RNA consists of exon4-exon3-exon4, 500bp: circular RNA onsists of exon4-exon5-exon6-exon2-exon3-exon4 while 700bp: circular RNA consists of exon4-exon5-exon4-exon4.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]