Difference between revisions of "Part:BBa K1486003:Experience"

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<I>Fudan iGEM 2015</I>
 
<I>Fudan iGEM 2015</I>
 
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We developed a new part([http://https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
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We developed a new part([https://parts.igem.org/Part:BBa_K1777005 BBa_K1777005]) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.
 
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Revision as of 12:47, 20 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1486003

User Reviews

UNIQ4d658ac1ef1d87ba-partinfo-00000000-QINU UNIQ4d658ac1ef1d87ba-partinfo-00000001-QINU UNIQ4d658ac1ef1d87ba-partinfo-00000002-QINU

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Fudan iGEM 2015

We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.

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