Difference between revisions of "Part:BBa K1675008"
| Line 4: | Line 4: | ||
L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD-dependent L-lactate dehydrogenase. The ''LdhA'' gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate. | L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD-dependent L-lactate dehydrogenase. The ''LdhA'' gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate. | ||
| − | To verified the'' LdhA'' gene, we constructed our gene into pET28a (Fig.1 | + | To verified the'' LdhA'' gene, we constructed our gene into pET28a (Fig.1), and induced its over-expression by IPTG (Isopropyl β-D-1-Thiogalactopyranoside) under 16 ℃ in BL21(DE)3. |
| − | |||
| − | |||
[[File:BIT_China_Regulation_System_pic26.jpg]] | [[File:BIT_China_Regulation_System_pic26.jpg]] | ||
| − | The constructed result of'' LdhA''-pET28a | + | Fig.1 The constructed result of'' LdhA''-pET28a |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:36, 20 September 2015
A device used in the synthesis of lactic acid
L-Lactic acid, one of the most important chiral molecules and organic acids, is produced via pyruvate from carbohydrates in diverse microorganisms catalyzed by an NAD-dependent L-lactate dehydrogenase. The LdhA gene is used in the synthesis of lactic acid. This gene encodes an L-lactate dehydrogenase (L-LDH) which converts pyruvate to L-lactate.
To verified the LdhA gene, we constructed our gene into pET28a (Fig.1), and induced its over-expression by IPTG (Isopropyl β-D-1-Thiogalactopyranoside) under 16 ℃ in BL21(DE)3.
Fig.1 The constructed result of LdhA-pET28a
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]