Difference between revisions of "Part:BBa K1806001"

Revision as of 16:05, 20 September 2015

Helicobacter pylori catalase

This part is the coding sequence for the Helicobacter pylori catalase, which effectively catalyses the breakdown of hydrogen peroxide. The breakdown of hydrogen peroxide, which is an exothermic process, will be utilized to provide heat to the medium to increase temperature to counteract a shift that decreases the temperature of the host environment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 852
Illegal BamHI site found at 1
Illegal XhoI site found at 1523
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This part is the coding sequence for the Helicobacter pylori catalase, which effectively catalyses the breakdown of hydrogen peroxide. The breakdown of hydrogen peroxide, which is an exothermic process, will be utilized to provide heat to the medium to increase temperature to counteract a shift that decreases the temperature of the host environment.
-== Cloning ==
-The part was ligated to the pSB1C3 cloning vector and the transformation was verified with Colony PCR. Afterwards, the isolates cut with EcoR1 and Pst1 were cutchecked. The functionality of the cloning of these parts can be seen in the gel images taken and placed below. The images on the right show the expected bands in the gel run.
-[[File:6001-1.png]] [[File:6001-2.png]]
-[[File:6001-3.png]] [[File:6001-4.png]]
 <!-- Add more about the biology of this part here