Difference between revisions of "Part:BBa K1616025"
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<partinfo>BBa_K1616025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1616025 SequenceAndFeatures</partinfo> | ||
+ | ===References=== | ||
+ | Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012 | ||
+ | <br>Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Latest revision as of 17:33, 19 September 2015
NanoLuc
NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
Thermal stable enzyme NanoLuc retains activity following 30 min incubation at 55 °C. Active over broad pH range NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. Monomeric enzyme NanoLuc facilitates use as transcriptional reporter or fusion partner.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc™ Luciferase Technology, 2012
Shreeram Akilesh, Bioluminescence: Nature’s Bright Idea,2000.