Difference between revisions of "Part:BBa K1616025"
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NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. | NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. | ||
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence. | NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence. | ||
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| + | Thermal stable enzyme | ||
| + | NanoLuc retains activity following 30 min incubation at 55 °C. | ||
| + | Active over broad pH range | ||
| + | NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. | ||
| + | Monomeric enzyme | ||
| + | NanoLuc facilitates use as transcriptional reporter or fusion partner. | ||
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| + | <center> | ||
| + | https://static.igem.org/mediawiki/2015/e/e6/NanoLuc.png | ||
| + | </center> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:32, 19 September 2015
NanoLuc
NanoLuc (Nluc)is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
Thermal stable enzyme NanoLuc retains activity following 30 min incubation at 55 °C. Active over broad pH range NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. Monomeric enzyme NanoLuc facilitates use as transcriptional reporter or fusion partner.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]