Difference between revisions of "Part:BBa J69511"

Revision as of 04:57, 20 September 2015

GFP reporter device - LacI repressible

Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct.

1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. These mutants were then characterise in Shewanella oneidensis MR1.

Our library entry can be found from BBa_K1676037 to BBa_K1676072 where GFP expression monitoring data can be found. :)

Results of the mutant library:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 725

@@ Line 4: / Line 4: @@
 Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct.
-AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases.
+AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. These mutants were then characterise in Shewanella oneidensis MR1.
-Our library entry can be found from BBa_K1676037 to BBa_K1676072.
+Our library entry can be found from BBa_K1676037 to BBa_K1676072 where GFP expression monitoring data can be found. :)
+https://static.igem.org/mediawiki/2015/4/49/MUTATIONS.png
 Results of the mutant library:
@@ Line 12: / Line 14: @@
 https://static.igem.org/mediawiki/2015/3/3a/Rbs-spots.jpeg
+https://static.igem.org/mediawiki/2015/9/90/Sumary.png