Difference between revisions of "Part:BBa K1629000"
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Liu, R. et. al. (May 2012) Beyond the detergent effect: a binding site for sodium dodecyl sulfate (SDS) in mammalian apoferritin. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/22525747 | Liu, R. et. al. (May 2012) Beyond the detergent effect: a binding site for sodium dodecyl sulfate (SDS) in mammalian apoferritin. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/22525747 | ||
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Latest revision as of 03:36, 19 September 2015
Light chain-heavy chain ferritin dimer with Histag 6X
Ferritin is an enzyme that captures iron ions in blood. It has been found that SDS molecules can bind between ferritin subunits. Although it's not the main function of this enzyme, team [http://2015.igem.org/Team:TecCEM_HS TecCEM_HS 2015] uses this enzyme to capture SDS molecules solution. This biobrick was based on a similar design from iGEM [http://2013.igem.org/Team:Calgary Team Calgary 2013].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1098
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 575
Illegal NgoMIV site found at 1040
Illegal NgoMIV site found at 1159
Illegal NgoMIV site found at 1223
Illegal NgoMIV site found at 1492 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 416
Illegal SapI.rc site found at 427
Illegal SapI.rc site found at 1540
Results
iGEM Groningen 2015 did an experiment with this construct to confirm it's expression. In the picture below is the expression of the protein visible. When comparing the bands in the control and the IPTG induction groups, a clear expression of a protein the size of ferritin can be observed.
