Difference between revisions of "Part:BBa K1807001:Experience"
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| + | Figure 1. Graph showing the phosphate concentration within the media for 5 different ppk variants and 1 ppx variant after 6 hours. Δppk Ecppk- native <i>Escherichia coli</i> ppk variant, Ecppx- native <i>Escherichia coli </i> ppx variant, Δ ppk is <i>Escherichia coli</i> with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - <i>Kingella oralis</i> ppk variant, PKDM12 <i>Escherichia coli</i> ppk variant from the Keasling collection. BW25113 is the parent <i>Escherichia coli</i> strain used in the experiment. One way ANOVA was used and overall there was no statistical difference between strains. Post-hoc LSD was also performed and there was statistical difference between Δppx and BW25113. * signifies there is p< 0.05.(ANOVA: F=1.616; d.f. = 17, p<0.229). Results were measured using a BMG FLUOstar Omega plate reader at OD<sub>650</sub> and concentrations calculated from known phosphate concentration standards. | ||
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| + | Figure 2. Graph showing the phosphate concentration of cell lysate of 10^8, cells for 5 different ppk variants and 1 ppx variant after 6 hours. ; Δppk Ecppk- native <i>Escherichia coli</i> ppk variant, Ecppx- native <i>Escherichia coli</i> ppx variant, Δ ppk is <i>Escherichia coli</i> with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - <i>Kingella oralis</i> ppk variant, pKDM12 <i>Escherichia coli</i> ppk variant from the Keasling collection. BW25113 is the parent <i>Escherichia coli</i> strain used for experiment. No statistical difference was shown. Results were measured using a BMG FLUOstar Omega plate reader at OD<sub>650</sub> and concentrations were calculated from known phosphate concentration standards. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:41, 19 September 2015
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Applications of BBa_K1807001
Figure 1. Graph showing the phosphate concentration within the media for 5 different ppk variants and 1 ppx variant after 6 hours. Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, PKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used in the experiment. One way ANOVA was used and overall there was no statistical difference between strains. Post-hoc LSD was also performed and there was statistical difference between Δppx and BW25113. * signifies there is p< 0.05.(ANOVA: F=1.616; d.f. = 17, p<0.229). Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations calculated from known phosphate concentration standards.
Figure 2. Graph showing the phosphate concentration of cell lysate of 10^8, cells for 5 different ppk variants and 1 ppx variant after 6 hours. ; Δppk Ecppk- native Escherichia coli ppk variant, Ecppx- native Escherichia coli ppx variant, Δ ppk is Escherichia coli with ppk knockout from the kanamycin insertion KEIO collection, Δppk Koppk - Kingella oralis ppk variant, pKDM12 Escherichia coli ppk variant from the Keasling collection. BW25113 is the parent Escherichia coli strain used for experiment. No statistical difference was shown. Results were measured using a BMG FLUOstar Omega plate reader at OD650 and concentrations were calculated from known phosphate concentration standards.
User Reviews
UNIQ75b8cd22ede2a48b-partinfo-00000002-QINU UNIQ75b8cd22ede2a48b-partinfo-00000003-QINU