Difference between revisions of "Part:BBa K1796007"

Revision as of 02:29, 19 September 2015

nifB from Paenibacillus sp. WLY78

essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1156
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1156
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1156
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1156
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1156
Illegal AgeI site found at 129
Illegal AgeI site found at 1089
Illegal AgeI site found at 1444
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1261

Parameter of Protein

Number of amino acids: 499

Molecular weight: 54869.8

Theoretical pI: 6.64

Amino acid composition:

Ala (A) 46 9.2%

Arg (R) 35 7.0%

Asn (N) 17 3.4%

Asp (D) 23 4.6%

Cys (C) 16 3.2%

Gln (Q) 19 3.8%

Glu (E) 40 8.0%

Gly (G) 42 8.4%

His (H) 17 3.4%

Ile (I) 30 6.0%

Leu (L) 41 8.2%

Lys (K) 25 5.0%

Met (M) 11 2.2%

Phe (F) 13 2.6%

Pro (P) 25 5.0%

Ser (S) 27 5.4%

Thr (T) 17 3.4%

Trp (W) 2 0.4%

Tyr (Y) 13 2.6%

Val (V) 40 8.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 63

Total number of positively charged residues (Arg + Lys): 60

Atomic composition:Carbon C 2398

Hydrogen H 3849

Nitrogen N 701

Oxygen O 719

Sulfur S 27

Formula: C2398H3849N701O719S27Total number of atoms: 7694

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 31370

Abs 0.1% (=1 g/l) 0.572, assuming all pairs of Cys residues form cystines

Ext. coefficient 30370

Abs 0.1% (=1 g/l) 0.553, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 43.03

This classifies the protein as unstable.

Aliphatic index: 87.96

Grand average of hydropathicity (GRAVY): -0.253

Aliphatic index: 92.50

Grand average of hydropathicity (GRAVY): -0.161

@@ Line 24: / Line 24: @@
 <p>	Theoretical pI: 6.64			</p>
 <p>				</p>
-<p>	Amino acid composition:聽			</p>
+<p>	Amino acid composition:			</p>
 <p>				</p>
 <p>	Ala (A)  46       9.2%			</p>