Difference between revisions of "Part:BBa K1602026"
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| − | This part is capable of generating the <b> green fluorescent protein (GFP) </b> under the control of a T7 promotor while codon optimized for usage in <i>E.coli</i> (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the proof of functional silica surface binding of the Si4tag. | + | This part is capable of generating the <b> green fluorescent protein (GFP) </b> under the control of a T7 promotor while codon optimized for usage in <i>E.coli</i> (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>). Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(<a href:"https://parts.igem.org/Part:BBa_K1028000">BBa_K1028000</a>) to the 3' end of the sequence, enables the proof of functional silica surface binding of the Si4tag. |
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Latest revision as of 02:13, 19 September 2015
Inducible generator of HisTag-GFP_Si4-Tag fusion protein
| This part is capable of generating the green fluorescent protein (GFP) under the control of a T7 promotor while codon optimized for usage in E.coli (BBa_B0034). Also the sequence of a Histag is fused to the gene sequence, improving the purification yield. Fusing the Si4-tag(BBa_K1028000) to the 3' end of the sequence, enables the proof of functional silica surface binding of the Si4tag. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 711