Difference between revisions of "Part:BBa K1820020"
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===Usage and Biology===
 
===Usage and Biology===
  
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<p> Transcption form this promoter is induced after phage infection of ''Lactococcus lactis'', resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in ''L. lactis''). Allowing for fluorescent detection of Φ31 phage infection in ''''Italic text''L. lactis''.</p>
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<p> Transcption form this promoter is induced after phage infection of ''Lactococcus lactis'', resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in ''L. lactis''). Allowing for fluorescent detection of Φ31 phage infection in ''L. lactis''.</p>
  
 
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Revision as of 01:04, 19 September 2015

P335-Φ31 inducible middle promoter + RBS+ sfGFP + term

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31 with the native ribosomal binding site (RBS), a super-folded green fluorescent protein (sfGFP) that was codon optimized for Bacillus subtilis (BBa_K1820004), and a transcriptional terminator.

Usage and Biology

Transcption form this promoter is induced after phage infection of Lactococcus lactis, resulting in expression of sfGFP (previously shown to have a high level of fluorescent activity in L. lactis). Allowing for fluorescent detection of Φ31 phage infection in L. lactis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42
    Illegal SapI.rc site found at 284