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− | <partinfo>BBa_K1640019 short</partinfo>
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− | ===Usage and Biology===
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− | ChlH is the catalytic subunit of Magnesium chelatase. This oligomeric enzyme initiates the first committed step of the chlorophyll-a biosynthesis pathway via insertion of an Mg2+ ion into protoporphyrin IX to generate Mg-protoporphyrin IX. Specifically, ChlH is the subunit known to bind porphyrin, and potentially also the Mg2+ ion. During this process, ChlH interacts with two AAA ATPase-like subunits of Mg-chelatase (ChlI and ChlD) to catalyse the ATP-dependent insertion of Mg2+ into protoporphyrin IX
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− | <p>The 4166 bp ChlH gene was engineered synthetically by Integrated DNA Technologies (IDT) in 3 gene blocks (Table 1). The original gene sequence was taken from <i>Chlamydomonas Reinhardtii</i> and subsequently codon optimized for expression in <i>Escherichia coli.</i> Integrity of the protein sequence was closely maintained throughout this optimisation process, but translation of the original clone and the synthesised sequences has revealed one mutation (‘E’ → ‘D’; ‘GAG’ → ‘GAT’). </p>
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| <span class='h3bb'>Sequence and Features</span> | | <span class='h3bb'>Sequence and Features</span> |
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| <partinfo>BBa_K1640019 parameters</partinfo> | | <partinfo>BBa_K1640019 parameters</partinfo> |
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| + | ===Overview=== |
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| + | ===Biology & Literature=== |
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| + | ===Protein information=== |
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| + | ===References=== |
Revision as of 01:03, 19 September 2015
Sequence and Features
No part name specified with partinfo tag.
Overview
Biology & Literature
Protein information
References