Difference between revisions of "Part:BBa K1728017:Design"

(References)
(Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
IL1β toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the downstream.
+
IL1β toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.
  
 
===References===
 
===References===
 
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939
 
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939

Latest revision as of 01:17, 19 September 2015


IL1β toehold switch RNA sensor with T7 promoter & luciferase reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 922


Design Notes

No.


Source

IL1β toehold switch RNA sensor with T7 promoter (order from synthesis company) assembled with luciferase reporter (BBa_I712019) at the upstream.

References

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin Toehold Switches: De-Novo-Designed Regulators of Gene Expression Cell.2014;159:925-939