Difference between revisions of "Part:BBa K1813013"

 
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<partinfo>BBa_K1813013 short</partinfo>
 
<partinfo>BBa_K1813013 short</partinfo>
  
A construct used to achieve the overexpression of HUMCYPDB1/CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host.
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A construct used to achieve the overexpression of CYP2D6 (<html><b><a href="https://parts.igem.org/Part:BBa_K1813005">BBa_K11813005</b></a></html>) in a bacterial host.
 
Structure: ptac:: HUMCYPDB1:: term.
 
Structure: ptac:: HUMCYPDB1:: term.
  
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<h2>Background</h2>
 
<h2>Background</h2>
Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of HUMCYPDB1 by a bacterial organism harboring this construct.
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Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP2D6 by a bacterial organism harboring this construct.
 
This construct was assembled by Michael VanInsberghe, and is codon optimized for <i> E. coli </i>. <br>
 
This construct was assembled by Michael VanInsberghe, and is codon optimized for <i> E. coli </i>. <br>
 
It is used in <html><b><a href="https://parts.igem.org/Part:BBa_K1813024">BBa_K1813024</b></a></html> and <html><b><a href="https://parts.igem.org/Part:BBa_K1813032">BBa_K1813032</b></a></html>.
 
It is used in <html><b><a href="https://parts.igem.org/Part:BBa_K1813024">BBa_K1813024</b></a></html> and <html><b><a href="https://parts.igem.org/Part:BBa_K1813032">BBa_K1813032</b></a></html>.

Latest revision as of 00:42, 19 September 2015

CYP2D6 Expression Cassette

A construct used to achieve the overexpression of CYP2D6 (BBa_K11813005) in a bacterial host. Structure: ptac:: HUMCYPDB1:: term.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]



Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP2D6 by a bacterial organism harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813024 and BBa_K1813032.