Difference between revisions of "Part:BBa K1714002"

Latest revision as of 23:44, 18 September 2015

pLac-RBS B0034-thanatin-double terminator

This part is designed for thanatin expression system regulated by pLac. By adding IPTG, this part expresses thanatin. Even we subcultured many colonies from LB plate to liquid culture, the number of correctly subcultured products were few. We expected that result could be caused by thanatin's strong toxicity. Expect naturally modified cells, which losing thanatin expression systems were not successed. It's very hard to survive with thanatin expression system even their toxicity was decreased by adding His10 and to get ideal sequences. We recommend you check whether this part's sequence is ideal sequence or not.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 233
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This part is designed for thanatin expression system regulated by pLac.
-By adding IPTG, this part express thanatin.
+By adding IPTG, this part expresses thanatin.
 Even we subcultured many colonies from LB plate to liquid culture, the number of correctly subcultured products were few.
-We expected that result could be caused by thanatin's strong toxicity.  Expect naturally modified cells, losing thanatin expression systems were not successed.
+We expected that result could be caused by thanatin's strong toxicity.  Expect naturally modified cells, which losing thanatin expression systems were not successed.
 It's very hard to survive with thanatin expression system even their toxicity was decreased by adding His10 and to get ideal sequences.
 We recommend you check whether this part's sequence is ideal sequence or not.