Difference between revisions of "Part:BBa K1585202"

Latest revision as of 23:18, 18 September 2015

PHI

6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E. coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate

Catalyzed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.

Usage and Biology

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:10, 18 September 2015 (view source) Pdemling (Talk \| contribs) ← Older edit		Latest revision as of 23:18, 18 September 2015 (view source) Pdemling (Talk \| contribs)
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−	6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate	+	6-Phospho-3-hexuloisomerase from ''Bacillus methanolicus'' MGA3, codon optimized for ''E. coli'', converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate