Difference between revisions of "Part:BBa K1585202"
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 23:10, 18 September 2015

PHI

6-Phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E. coli, converts D-Arabino-hex-3-ulose-6-phosphate to D-Fructose-6-phosphate


Catalyzed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.



Usage and Biology

Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]