Difference between revisions of "Part:BBa K1819000"

Revision as of 23:05, 18 September 2015

Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site.

It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.

Figure 1 - Schematic representation of BBa_K1819000 insert

Characterization

Figure 2 - Gel restriction analysis

We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see Experience page) .
This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 674

@@ Line 21: / Line 21: @@
-<p>We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see <a href="https://parts.igem.org/Part:BBa_E0040:Experience>"Experience page</a>) .
+<p>We added this part as an improvement to BBa_E0040, a green fluorescente protein, for protein fusion (see <a href="https://parts.igem.org/Part:BBa_E0040:Experience">Experience page</a>) .
 <br>
 This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.</p>