Difference between revisions of "Part:BBa K1614024"

Revision as of 19:32, 20 September 2015

Actin AptaBody with software generated actin aptamer (17mer)

Notice: Functional DNA

This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.

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MAWS software generated actin aptamer (17mer) linked with a 5x A linker to the horseradish peroxidase mimicking DNAzyme.

This part comprises an aptamer designed to targed actin using MAWS a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1614024 short</partinfo>
+{{Template:ssDNA}}</br>
+MAWS software generated actin aptamer (17mer) linked with a 5x A linker to the horseradish peroxidase mimicking DNAzyme.
-MAWS software generated actin aptamer (17mer) linked with a 5x A linker to the horseradish peroxidase mimmic DNAzyme.
+This part comprises an aptamer designed to targed actin using <b>MAWS</b> a linker of AAAAA and the HRP-DNAzyme. It is capable of providing luminescence readout in the presence of hydrogen peroxide, and designed for Western blotting. Degradation of hydrogen peroxide needed for luminescent readout with luminol requires the presence of hemin, with optimal behaviour at equimolar concentration of hemin and the construct. The optimal pH for the HRP-DNAzyme used in this construct is pH 8.5.