Difference between revisions of "Part:BBa K1761006:Experience"
Tjvsonsbeek (Talk | contribs) |
|||
| Line 6: | Line 6: | ||
| − | == Application TU Eindhoven == | + | == Application TU Eindhoven 2015 == |
SmallBit is used as an intracellulair signaling domain. In combination with LargeBit [https://parts.igem.org/Part:BBa_K1761005] a Bioluminescence signal can be generated by adding furimazine. We verified that this part alone with furimazine gives no signal. The same goes for SmallBit. Therefore, these parts are ideally as signaling components if you want to check if two parts are present. | SmallBit is used as an intracellulair signaling domain. In combination with LargeBit [https://parts.igem.org/Part:BBa_K1761005] a Bioluminescence signal can be generated by adding furimazine. We verified that this part alone with furimazine gives no signal. The same goes for SmallBit. Therefore, these parts are ideally as signaling components if you want to check if two parts are present. | ||
| + | |||
| + | == Application TU Eindhoven 2016 == | ||
| + | |||
| + | iGEM TU Eindhoven used this part in combination with LargeBiT ("https://parts.igem.org/Part:BBa_K1761005"). These parts were linked to the CT52 protein. These linked parts are: <br> CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007" <br>Those two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application the NanoBiT system visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out". | ||
| + | |||
| + | [[File:T--TU-Eindhoven--NanolUcreadoutbb.png]] | ||
| + | <br> | ||
| + | ''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52'' | ||
| + | |||
| + | |||
Revision as of 09:40, 19 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1761006
Application TU Eindhoven 2015
SmallBit is used as an intracellulair signaling domain. In combination with LargeBit [1] a Bioluminescence signal can be generated by adding furimazine. We verified that this part alone with furimazine gives no signal. The same goes for SmallBit. Therefore, these parts are ideally as signaling components if you want to check if two parts are present.
Application TU Eindhoven 2016
iGEM TU Eindhoven used this part in combination with LargeBiT ("https://parts.igem.org/Part:BBa_K1761005"). These parts were linked to the CT52 protein. These linked parts are:
CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007"
Those two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. This system was used as a read out method for T14-3-3 heterodimers. It is schemtically visualised in figure 1. For more detailed information about the application the NanoBiT system visit our wiki "http://2016.igem.org/Team:TU-Eindhoven/Read-out".
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52
User Reviews
UNIQe07a6e72181b98df-partinfo-00000000-QINU UNIQe07a6e72181b98df-partinfo-00000001-QINU