Difference between revisions of "Part:BBa K1614001"
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<partinfo>BBa_K1614001 short</partinfo>
 
<partinfo>BBa_K1614001 short</partinfo>
  
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{{Template:ssDNA}}<br/>
 
  
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The hepatitis delta virus ribozyme shows a specific and highly efficient cleavage activity at the RNAs 5' end of the sequence. Therefore it is highly suitable to create defined ends for functional RNA expression.
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The hepatitis delta virus ribozyme is a self cleaving ribozyme originating from hepatitis delta virus. The cleavage occurs at the ribozymes 5' end independent from the upstream sequence. Therefore it is highly suitable to create defined ends for functional RNA expression. Furthermore its behvior is specific and shows good efficiency.
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Therefore we used this ribozyme for all BBF RFC 110 parts, as the 3' limiter. Thereby the production of RNA product with the user-defined ends is ensured. For more details have a look at our cloning and transcription cassette <br/>
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[[File:https://static.igem.org/mediawiki/parts/e/e1/HDV_Cleavage_Behavior.png"|!!!Fig.1: PAGE analysis of HDV cleavage behavior.!!! The RNA of interest appears as a defined band under UV-shadowing. Higher yields for the desired product with high fidelity can be obtained by the PCR extraction of the [https://parts.igem.org/Part:BBa_K1614002|transcription cassette] with [https://parts.igem.org/Part:BBa_K1614005|BBa_K1614005] [https://parts.igem.org/Part:BBa_K1614006|BBa_K1614006]. Uncleaved product can be observed as the third band.]]
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1614001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1614001 SequenceAndFeatures</partinfo>

Revision as of 23:18, 20 September 2015

Hepatitis Delta Virus(HDV) Ribozyme


The hepatitis delta virus ribozyme is a self cleaving ribozyme originating from hepatitis delta virus. The cleavage occurs at the ribozymes 5' end independent from the upstream sequence. Therefore it is highly suitable to create defined ends for functional RNA expression. Furthermore its behvior is specific and shows good efficiency.

Therefore we used this ribozyme for all BBF RFC 110 parts, as the 3' limiter. Thereby the production of RNA product with the user-defined ends is ensured. For more details have a look at our cloning and transcription cassette
!!!Fig.1: PAGE analysis of HDV cleavage behavior.!!! The RNA of interest appears as a defined band under UV-shadowing. Higher yields for the desired product with high fidelity can be obtained by the PCR extraction of the cassette with [1] [2]. Uncleaved product can be observed as the third band.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]