Difference between revisions of "Part:BBa K1725103"
 
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===Usage and Biology===
 
===Usage and Biology===
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This is a reporter plasmid that expresses RFP from the SrpR regulated promoter <bbpart>BBa_xxx</bbpart> and expresses gfp from the PhlF regulated promoter <bbpart>BBa_xxx</bbpart> <BR>
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This is a reporter plasmid that expresses RFP from the SrpR regulated promoter <bbpart>BBa_K1725020</bbpart> and expresses gfp from the PhlF regulated promoter <bbpart>BBa_K1725000</bbpart> <BR>
  
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GFP will be off in the presence of SrpR biobrick <bbpart>BBa_xxx</bbpart> while RFP will be off in the presence of the PhlF biobrick <bbpart>BBa_xxx</bbpart>
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GFP will be off in the presence of the SrpR biobrick <bbpart>BBa_K1725060</bbpart> while RFP will be off in the presence of the PhlF biobrick <bbpart>BBa_K1725040</bbpart>
  
 
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Latest revision as of 22:12, 18 September 2015

SrpR repressible promoter + strong RBS + RFP + terminator + PhlF repressible promoter + weak RBS + G

.


Usage and Biology

This is a reporter plasmid that expresses RFP from the SrpR regulated promoter BBa_K1725020 and expresses gfp from the PhlF regulated promoter BBa_K1725000

GFP will be off in the presence of the SrpR biobrick BBa_K1725060 while RFP will be off in the presence of the PhlF biobrick BBa_K1725040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 651
    Illegal AgeI site found at 763
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1666