Difference between revisions of "Part:BBa K1813009"
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<partinfo>BBa_K1813009 parameters</partinfo>
 
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<h2>Background of <i>nicF</i>:</h2>
 
<h2>Background of <i>nicF</i>:</h2>

Revision as of 22:12, 18 September 2015

nicF

nicF Gen Bank accession number Q88FY5.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 439
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicF:

Part Description:

Codon optimized coding sequence of nicF, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813017 and BBa_K1813023 .

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicF converts Maleamic Acid to Maleic Acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105