Difference between revisions of "Part:BBa K1648048"
| Line 3: | Line 3: | ||
[[File:K1648048_Map.png|350px|thumb|right|Construct Map for K1648048]] | [[File:K1648048_Map.png|350px|thumb|right|Construct Map for K1648048]] | ||
| + | |||
| + | |||
<div style="text-align:justify; text-justify:inter-ideograph"> | <div style="text-align:justify; text-justify:inter-ideograph"> | ||
| Line 8: | Line 10: | ||
OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. '''BBa_K1648048 provides constitutive transcription of oprF of ''Azotobacter vinelandii''.''' | OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. '''BBa_K1648048 provides constitutive transcription of oprF of ''Azotobacter vinelandii''.''' | ||
| − | [[File:K1648048_dd.png|350px|thumb| | + | |
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | [[File:K1648048_dd.png|350px|thumb|right|Double digestion of K1648048: L: DNA ladder. Lane 1-3: Recombination Template for J13002-oprF(BBa_K1648048) with double digestion cut at EcoR1 and PstI sites, with single digestion at Pst1 site, without digestion.]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 22:12, 18 September 2015
AvOprF induced by J13002
OprF is a transmembrane porin which allows non-specific transmission. For constructing an efficient microbial fuel cell(MFC), we manipulated the expression of oprF. oprF allows electron carriers shuttle in and out of the bacteria, the electron generator. The electrons generated by the bacteria are taken up by the incoming carrier. Reduced electron carriers transport the electrons out of the bacteria to the electrode. BBa_K1648048 provides constitutive transcription of oprF of Azotobacter vinelandii.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 555