Difference between revisions of "Part:BBa K1813007"
Line 25: Line 25:
  
 
<h4>Part Function:</h4>
 
<h4>Part Function:</h4>
−
<p>The enzyme coded for by <i>nicD</i> converts N-FormylMaleamic (NFM) to Maleamic Acid (2,5-DHP).</p>
+
<p>The enzyme coded for by <i>nicD</i> converts N-FormylMaleamic (NFM) to Maleamic Acid.</p>
  
 
<h4>References:</h4>
 
<h4>References:</h4>
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Revision as of 21:41, 18 September 2015

nicD

nicD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of nicD:

Part Description:

Codon optimized coding sequence of nicD, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813007.

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicD converts N-FormylMaleamic (NFM) to Maleamic Acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105